| Model | EF100mm f/2.8 Macro USM | EF100mm f/2.8L Macro IS USM |
| Angle of View (Diagonal) | 24° | 19.8° |
| Angle of View (Horizontal) | 20° | 23.4° |
| Angle of View (Vertical) | 14° | 13.5° |
| Closest Focusing Distance (m, ft) | 0.31/1.0 | 0.3/0.99 |
| Construction (groups elements) | 8-12 | 12-15 |
| Diameter x Length (mm) (Maximum) | 78.6 x 118.6 | 77.7 x 123 |
| Distance Scale | o | o |
| Drive System | Ring-type USM | Ring-type USM |
| Filter Size (mm) | 58 | 67 |
| Maximum Magnification (x) | 1 | 1 |
| Minimum Aperture | 32 | 2.8 |
| No. of Diaphragm Blades | 8 | 9 |
| Water or dust-resistance | - | o |
| Weight (g) (Approximately) | 580 | 625 |
| With Extender EF1.4XII attached | ||
| AF | - | - |
| Focal length (mm) | - | - |
| IS | - | - |
| Maximum aperture (f) | - | - |
| Maximum magnification (x) | - | - |
| With Extender EF2XII attached | ||
| AF | - | - |
| Focal length (mm) | - | - |
| IS | - | - |
| Maximum aperture (f) | - | - |
| Maximum magnification (x) | - | - |
| Accessories | ||
| Extension Tube EF12 II (Optional) | 1.19~0.12X | - |
| Extension Tube EF25 II (Optional) | 1.39~0.26X | - |
| Gelatin Filter Holder III (Hood III) | 4 | - |
| Gelatin Filter Holder IV (Hood IV) | 4 | - |
| Lens Cap | E-58U | E-67U |
| Lens Hood | ET-67 | ET-73 |
| Lens Pouch or Case | LP1219 | LP1219 |